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KMID : 0613820070170081141
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Journal of Life Science
2007 Volume.17 No. 8 p.1141 ~ p.1146
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Studies on Antioxidant Activity and Inhibition of Nitric Oxide Synthesis of Germinated Brown Rice Soaked in Mycelial Culture Broth of Phellinus linteus
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Jung Il-Sun
Kim Yu-Jung
Choi In-Soon
Choi Eun-Young
Shin Su-Hwa
Gal Sang-Wan
Choi Young-Ju
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Abstract
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This study investigated the effects on the biological activities of germinated brown rice soaked in mycelial culture broth of Phellinus linteus. The level of free amino acid was higher in the GBRP extract than those of BR and GBR. The major free amino acids were alanine, valine, isoleucine and methionine in both extracts. The level of γ-aminobutyric acid (GABA) was also increased significantly in the GBR and GBRP. Antioxidant activities of methanol extract of BR, GBR and GBRP were measured by using DPPH radical scavenging and SOD-like activity. Antioxidant activities showed the highest level of 83% and 76% when 100 ㎎/㎖ GBR and GBRP, respectively. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the methanol extract of GBR and GBRP showed marked inhibition of NO synthesis in a does-dependant manner. These results showed that GBR and GBRP were significant role for activation of immune system in the pathogenesis of inflammatory diseases.
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KEYWORD
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Phellinus linteus, GABA, nitric oxide, germinated brown rice
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